Chemical-Free and Reusable Cellular Analysis: Electrochemical Impedance Spectroscopy with a Transparent ITO Culture Chip

Sheng-Yi Hsiao, Dai-Chian Chen, Chih-Hui Yang, Haw-Ming Huang, Yen-Pei Lu, Hui-Shun Huang, Chun-Yen Lin and Yung-Sheng Lin
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Sheng-Yi Hsiao: Instrument Technology Research Center, National Applied Research Laboratories, Taiwan
Dai-Chian Chen: National Yang-Ming University, Taiwan
Chih-Hui Yang: I-Shou University, Taiwan
Haw-Ming Huang: Taipei Medical University, Taiwan
Yen-Pei Lu: Instrument Technology Research Center, National Applied Research Laboratories, Taiwan
Hui-Shun Huang: Instrument Technology Research Center, National Applied Research Laboratories, Taiwan
Chun-Yen Lin: Taipei Medical University, Taiwan
Yung-Sheng Lin: Hungkuang University, Taiwan

International Journal of Technology and Human Interaction (IJTHI), 2012, vol. 8, issue 3, 1-9

Abstract: To advance innovative green technology in studying cytology, this study developed an electrochemical impedance spectroscopic (EIS) system with an indium tin oxide (ITO) culture chip module. This paper also demonstrates typical examples of solution effects and B16-F10 cell culture. Results indicate that higher concentrations of saline or albumin had lower impedance. From impedance data, cell proliferation and decline could be elucidated. The impedance soon decreased when Triton X-100 was applied to kill cells. Furthermore, the implemented transparent ITO culture chip module is experiment-friendly to perform optical inspections. The proposed green EIS system which is advantage of chemical-free and reusability can be widely applied to cytology studies in the future.

Date: 2012
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