Single cell RNA analysis identifies cellular heterogeneity and adaptive responses of the lung at birth

Minzhe Guo, Yina Du, Jason J. Gokey, Samriddha Ray, Sheila M. Bell, Mike Adam, Parvathi Sudha, Anne Karina Perl, Hitesh Deshmukh, S. Steven Potter, Jeffrey A. Whitsett () and Yan Xu ()
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Minzhe Guo: Cincinnati Children’s Hospital Medical Center
Yina Du: Cincinnati Children’s Hospital Medical Center
Jason J. Gokey: Cincinnati Children’s Hospital Medical Center
Samriddha Ray: Cincinnati Children’s Hospital Medical Center
Sheila M. Bell: Cincinnati Children’s Hospital Medical Center
Mike Adam: Cincinnati Children’s Hospital Medical Center
Parvathi Sudha: Cincinnati Children’s Hospital Medical Center
Anne Karina Perl: Cincinnati Children’s Hospital Medical Center
Hitesh Deshmukh: Cincinnati Children’s Hospital Medical Center
S. Steven Potter: Cincinnati Children’s Hospital Medical Center
Jeffrey A. Whitsett: Cincinnati Children’s Hospital Medical Center
Yan Xu: Cincinnati Children’s Hospital Medical Center

Nature Communications, 2019, vol. 10, issue 1, 1-16

Abstract: Abstract The respiratory system undergoes a diversity of structural, biochemical, and functional changes necessary for adaptation to air breathing at birth. To identify the heterogeneity of pulmonary cell types and dynamic changes in gene expression mediating adaptation to respiration, here we perform single cell RNA analyses of mouse lung on postnatal day 1. Using an iterative cell type identification strategy we unbiasedly identify the heterogeneity of murine pulmonary cell types. We identify distinct populations of epithelial, endothelial, mesenchymal, and immune cells, each containing distinct subpopulations. Furthermore we compare temporal changes in RNA expression patterns before and after birth to identify signaling pathways selectively activated in specific pulmonary cell types, including activation of cell stress and the unfolded protein response during perinatal adaptation of the lung. The present data provide a single cell view of the adaptation to air breathing after birth.

Date: 2019
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DOI: 10.1038/s41467-018-07770-1

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