Acto-myosin force organization modulates centriole separation and PLK4 recruitment to ensure centriole fidelity

Elisa Vitiello (), Philippe Moreau, Vanessa Nunes, Amel Mettouchi, Helder Maiato, Jorge G. Ferreira, Irène Wang and Martial Balland
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Elisa Vitiello: Domaine universitaire
Philippe Moreau: Domaine universitaire
Vanessa Nunes: Universidade do Porto
Amel Mettouchi: Institut Pasteur, Département de Microbiologie, Unité des Toxines Bactériennes, Université Paris Descartes
Helder Maiato: Universidade do Porto
Jorge G. Ferreira: Universidade do Porto
Irène Wang: Domaine universitaire
Martial Balland: Domaine universitaire

Nature Communications, 2019, vol. 10, issue 1, 1-12

Abstract: Abstract The presence of aberrant number of centrioles is a recognized cause of aneuploidy and hallmark of cancer. Hence, centriole duplication needs to be tightly regulated. It has been proposed that centriole separation limits centrosome duplication. The mechanism driving centriole separation is poorly understood and little is known on how this is linked to centriole duplication. Here, we propose that actin-generated forces regulate centriole separation. By imposing geometric constraints via micropatterns, we were able to prove that precise acto-myosin force arrangements control direction, distance and time of centriole separation. Accordingly, inhibition of acto-myosin contractility impairs centriole separation. Alongside, we observed that organization of acto-myosin force modulates specifically the length of S-G2 phases of the cell cycle, PLK4 recruitment at the centrosome and centriole fidelity. These discoveries led us to suggest that acto-myosin forces might act in fundamental mechanisms of aneuploidy prevention.

Date: 2019
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DOI: 10.1038/s41467-018-07965-6

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