Coordinative metabolism of glutamine carbon and nitrogen in proliferating cancer cells under hypoxia

Yuanyuan Wang, Changsen Bai, Yuxia Ruan, Miao Liu, Qiaoyun Chu, Li Qiu, Chuanzhen Yang and Binghui Li ()
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Yuanyuan Wang: Tianjin Medical University Cancer Institute and Hospital
Changsen Bai: Tianjin Medical University Cancer Institute and Hospital
Yuxia Ruan: Tianjin Medical University Cancer Institute and Hospital
Miao Liu: Tianjin Medical University Cancer Institute and Hospital
Qiaoyun Chu: Capital Medical University
Li Qiu: Tianjin Medical University Cancer Institute and Hospital
Chuanzhen Yang: Capital Medical University
Binghui Li: Tianjin Medical University Cancer Institute and Hospital

Nature Communications, 2019, vol. 10, issue 1, 1-14

Abstract: Abstract Under hypoxia, most of glucose is converted to secretory lactate, which leads to the overuse of glutamine-carbon. However, under such a condition how glutamine nitrogen is disposed to avoid over-accumulating ammonia remains to be determined. Here we identify a metabolic flux of glutamine to secretory dihydroorotate, which is indispensable to glutamine-carbon metabolism under hypoxia. We found that glutamine nitrogen is necessary to nucleotide biosynthesis, but enriched in dihyroorotate and orotate rather than processing to its downstream uridine monophosphate under hypoxia. Dihyroorotate, not orotate, is then secreted out of cells. Furthermore, we found that the specific metabolic pathway occurs in vivo and is required for tumor growth. The identified metabolic pathway renders glutamine mainly to acetyl coenzyme A for lipogenesis, with the rest carbon and nitrogen being safely removed. Therefore, our results reveal how glutamine carbon and nitrogen are coordinatively metabolized under hypoxia, and provide a comprehensive understanding on glutamine metabolism.

Date: 2019
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DOI: 10.1038/s41467-018-08033-9

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