The cell cycle regulator GpsB functions as cytosolic adaptor for multiple cell wall enzymes

Robert M. Cleverley, Zoe J. Rutter, Jeanine Rismondo, Federico Corona, Ho-Ching Tiffany Tsui, Fuad A. Alatawi, Richard A. Daniel, Sven Halbedel, Orietta Massidda, Malcolm E. Winkler and Richard J. Lewis ()
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Robert M. Cleverley: University of Newcastle
Zoe J. Rutter: University of Newcastle
Jeanine Rismondo: Robert Koch Institute
Federico Corona: Università di Cagliari
Ho-Ching Tiffany Tsui: Indiana University Bloomington
Fuad A. Alatawi: University of Newcastle
Richard A. Daniel: University of Newcastle
Sven Halbedel: Robert Koch Institute
Orietta Massidda: Università di Cagliari
Malcolm E. Winkler: Indiana University Bloomington
Richard J. Lewis: University of Newcastle

Nature Communications, 2019, vol. 10, issue 1, 1-17

Abstract: Abstract Bacterial growth and cell division requires precise spatiotemporal regulation of the synthesis and remodelling of the peptidoglycan layer that surrounds the cytoplasmic membrane. GpsB is a cytosolic protein that affects cell wall synthesis by binding cytoplasmic mini-domains of peptidoglycan synthases to ensure their correct subcellular localisation. Here, we describe critical structural features for the interaction of GpsB with peptidoglycan synthases from three bacterial species (Bacillus subtilis, Listeria monocytogenes and Streptococcus pneumoniae) and suggest their importance for cell wall growth and viability in L. monocytogenes and S. pneumoniae. We use these structural motifs to identify novel partners of GpsB in B. subtilis and extend the members of the GpsB interactome in all three bacterial species. Our results support that GpsB functions as an adaptor protein that mediates the interaction between membrane proteins, scaffolding proteins, signalling proteins and enzymes to generate larger protein complexes at specific sites in a bacterial cell cycle-dependent manner.

Date: 2019
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DOI: 10.1038/s41467-018-08056-2

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