Strigolactone perception and deactivation by a hydrolase receptor DWARF14

Yoshiya Seto (), Rei Yasui, Hiromu Kameoka, Muluneh Tamiru, Mengmeng Cao, Ryohei Terauchi, Akane Sakurada, Rena Hirano, Takaya Kisugi, Atsushi Hanada, Mikihisa Umehara, Eunjoo Seo, Kohki Akiyama, Jason Burke, Noriko Takeda-Kamiya, Weiqiang Li, Yoshinori Hirano, Toshio Hakoshima, Kiyoshi Mashiguchi, Joseph P. Noel, Junko Kyozuka and Shinjiro Yamaguchi ()
Additional contact information
Yoshiya Seto: Tohoku University
Rei Yasui: Tohoku University
Hiromu Kameoka: University of Tokyo, Yayoi, Bunkyo
Muluneh Tamiru: Iwate Biotechnology Research Center
Mengmeng Cao: Tohoku University
Ryohei Terauchi: Iwate Biotechnology Research Center
Akane Sakurada: Tohoku University
Rena Hirano: Tohoku University
Takaya Kisugi: Tohoku University
Atsushi Hanada: Tohoku University
Mikihisa Umehara: RIKEN Plant Science Center, Tsurumi
Eunjoo Seo: RIKEN Plant Science Center, Tsurumi
Kohki Akiyama: Osaka Prefecture University
Jason Burke: Salk Institute for Biological Studies
Noriko Takeda-Kamiya: RIKEN Plant Science Center, Tsurumi
Weiqiang Li: RIKEN Plant Science Center, Tsurumi
Yoshinori Hirano: Nara Institute of Science and Technology
Toshio Hakoshima: Nara Institute of Science and Technology
Kiyoshi Mashiguchi: Tohoku University
Joseph P. Noel: Salk Institute for Biological Studies
Junko Kyozuka: Tohoku University
Shinjiro Yamaguchi: Tohoku University

Nature Communications, 2019, vol. 10, issue 1, 1-10

Abstract: Abstract The perception mechanism for the strigolactone (SL) class of plant hormones has been a subject of debate because their receptor, DWARF14 (D14), is an α/β-hydrolase that can cleave SLs. Here we show via time-course analyses of SL binding and hydrolysis by Arabidopsis thaliana D14, that the level of uncleaved SL strongly correlates with the induction of the active signaling state. In addition, we show that an AtD14D218A catalytic mutant that lacks enzymatic activity is still able to complement the atd14 mutant phenotype in an SL-dependent manner. We conclude that the intact SL molecules trigger the D14 active signaling state, and we also describe that D14 deactivates bioactive SLs by the hydrolytic degradation after signal transmission. Together, these results reveal that D14 is a dual-functional receptor, responsible for both the perception and deactivation of bioactive SLs.

Date: 2019
References: Add references at CitEc
Citations: View citations in EconPapers (4)

Downloads: (external link)
https://www.nature.com/articles/s41467-018-08124-7 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-018-08124-7

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-018-08124-7

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().