Anti-CRISPR-mediated control of gene editing and synthetic circuits in eukaryotic cells

Muneaki Nakamura, Prashanth Srinivasan, Michael Chavez, Matthew A. Carter, Antonia A. Dominguez, Marie La Russa, Matthew B. Lau, Timothy R. Abbott, Xiaoshu Xu, Dehua Zhao, Yuchen Gao, Nathan H. Kipniss, Christina D. Smolke, Joseph Bondy-Denomy and Lei S. Qi ()
Additional contact information
Muneaki Nakamura: Stanford University
Prashanth Srinivasan: Stanford University
Michael Chavez: Stanford University
Matthew A. Carter: Stanford University
Antonia A. Dominguez: Stanford University
Marie La Russa: Stanford University
Matthew B. Lau: Stanford University
Timothy R. Abbott: Stanford University
Xiaoshu Xu: Stanford University
Dehua Zhao: Stanford University
Yuchen Gao: Stanford University
Nathan H. Kipniss: Stanford University
Christina D. Smolke: Stanford University
Joseph Bondy-Denomy: University of California
Lei S. Qi: Stanford University

Nature Communications, 2019, vol. 10, issue 1, 1-11

Abstract: Abstract Repurposed CRISPR-Cas molecules provide a useful tool set for broad applications of genomic editing and regulation of gene expression in prokaryotes and eukaryotes. Recent discovery of phage-derived proteins, anti-CRISPRs, which serve to abrogate natural CRISPR anti-phage activity, potentially expands the ability to build synthetic CRISPR-mediated circuits. Here, we characterize a panel of anti-CRISPR molecules for expanded applications to counteract CRISPR-mediated gene activation and repression of reporter and endogenous genes in various cell types. We demonstrate that cells pre-engineered with anti-CRISPR molecules become resistant to gene editing, thus providing a means to generate “write-protected” cells that prevent future gene editing. We further show that anti-CRISPRs can be used to control CRISPR-based gene regulation circuits, including implementation of a pulse generator circuit in mammalian cells. Our work suggests that anti-CRISPR proteins should serve as widely applicable tools for synthetic systems regulating the behavior of eukaryotic cells.

Date: 2019
References: Add references at CitEc
Citations: View citations in EconPapers (2)

Downloads: (external link)
https://www.nature.com/articles/s41467-018-08158-x Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-018-08158-x

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-018-08158-x

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().