Efficient oral vaccination by bioengineering virus-like particles with protozoan surface proteins

Marianela C. Serradell, Lucía L. Rupil, Román A. Martino, César G. Prucca, Pedro G. Carranza, Alicia Saura, Elmer A. Fernández, Pablo R. Gargantini, Albano H. Tenaglia, Juan P. Petiti, Renata R. Tonelli, Nicolás Reinoso-Vizcaino, José Echenique, Luciana Berod, Eliane Piaggio, Bertrand Bellier, Tim Sparwasser, David Klatzmann and Hugo D. Luján ()
Additional contact information
Marianela C. Serradell: Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)/Universidad Católica de Córdoba (UCC)
Lucía L. Rupil: Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)/Universidad Católica de Córdoba (UCC)
Román A. Martino: Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)/Universidad Católica de Córdoba (UCC)
César G. Prucca: Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)/Universidad Católica de Córdoba (UCC)
Pedro G. Carranza: Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)/Universidad Católica de Córdoba (UCC)
Alicia Saura: Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)/Universidad Católica de Córdoba (UCC)
Elmer A. Fernández: Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)/Universidad Católica de Córdoba (UCC)
Pablo R. Gargantini: Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)/Universidad Católica de Córdoba (UCC)
Albano H. Tenaglia: Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)/Universidad Católica de Córdoba (UCC)
Juan P. Petiti: CONICET/Universidad Nacional de Córdoba
Renata R. Tonelli: Universidade Federal de São Paulo
Nicolás Reinoso-Vizcaino: CONICET/Universidad Nacional de Córdoba
José Echenique: CONICET/Universidad Nacional de Córdoba
Luciana Berod: Hannover Medical School and Helmholtz Centre for Infection Research
Eliane Piaggio: Sorbonne Université, INSERM, Immunology-Immunopathology-Immunotherapy (i3), AP-HP, Hôpital Pitié-Salpêtrière, Biotherapy (CIC-BTi) and Inflammation-Immunopathology-Biotherapy Department (i2B)
Bertrand Bellier: Sorbonne Université, INSERM, Immunology-Immunopathology-Immunotherapy (i3), AP-HP, Hôpital Pitié-Salpêtrière, Biotherapy (CIC-BTi) and Inflammation-Immunopathology-Biotherapy Department (i2B)
Tim Sparwasser: Hannover Medical School and Helmholtz Centre for Infection Research
David Klatzmann: Sorbonne Université, INSERM, Immunology-Immunopathology-Immunotherapy (i3), AP-HP, Hôpital Pitié-Salpêtrière, Biotherapy (CIC-BTi) and Inflammation-Immunopathology-Biotherapy Department (i2B)
Hugo D. Luján: Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)/Universidad Católica de Córdoba (UCC)

Nature Communications, 2019, vol. 10, issue 1, 1-15

Abstract: Abstract Intestinal and free-living protozoa, such as Giardia lamblia, express a dense coat of variant-specific surface proteins (VSPs) on trophozoites that protects the parasite inside the host’s intestine. Here we show that VSPs not only are resistant to proteolytic digestion and extreme pH and temperatures but also stimulate host innate immune responses in a TLR-4 dependent manner. We show that these properties can be exploited to both protect and adjuvant vaccine antigens for oral administration. Chimeric Virus-like Particles (VLPs) decorated with VSPs and expressing model surface antigens, such as influenza virus hemagglutinin (HA) and neuraminidase (NA), are protected from degradation and activate antigen presenting cells in vitro. Orally administered VSP-pseudotyped VLPs, but not plain VLPs, generate robust immune responses that protect mice from influenza infection and HA-expressing tumors. This versatile vaccine platform has the attributes to meet the ultimate challenge of generating safe, stable and efficient oral vaccines.

Date: 2019
References: Add references at CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/s41467-018-08265-9 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-018-08265-9

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-018-08265-9

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().