The path of pre-ribosomes through the nuclear pore complex revealed by electron tomography

Franck Delavoie, Vanessa Soldan, Dana Rinaldi, Jean-Yves Dauxois and Pierre-Emmanuel Gleizes ()
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Franck Delavoie: Université de Toulouse, CNRS, UPS
Vanessa Soldan: Université de Toulouse, CNRS, UPS
Dana Rinaldi: Université de Toulouse, CNRS, UPS
Jean-Yves Dauxois: Université de Toulouse, CNRS, INSA
Pierre-Emmanuel Gleizes: Université de Toulouse, CNRS, UPS

Nature Communications, 2019, vol. 10, issue 1, 1-12

Abstract: Abstract Determining the path of single ribonucleoprotein (RNP) particles through the 100 nm-wide nuclear pore complex (NPC) by fluorescence microscopy remains challenging due to resolution limitation and RNP labeling constraints. By using high-pressure freezing and electron tomography, here we captured snapshots of the translocation of native RNP particles through NPCs in yeast and analyzed their trajectory at nanometer-scale resolution. Morphological and functional analyses indicate that these particles mostly correspond to pre-ribosomes. They are detected in 5–6% of the NPCs, with no apparent bias for NPCs adjacent to the nucleolus. Their path closely follows the central axis of the NPC through the nuclear and inner rings, but diverges at the cytoplasmic ring, suggesting interactions with the cytoplasmic nucleoporins. By applying a probabilistic queueing model to our data, we estimated that the dwell time of pre-ribosomes in the yeast NPC is ~90 ms. These data reveal distinct steps of pre-ribosome translocation through the NPC.

Date: 2019
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DOI: 10.1038/s41467-019-08342-7

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