Membrane reshaping by micrometric curvature sensitive septin filaments

Alexandre Beber, Cyntia Taveneau, Manuela Nania, Feng-Ching Tsai, Aurelie Cicco, Patricia Bassereau, Daniel Lévy, João T. Cabral, Hervé Isambert, Stéphanie Mangenot () and Aurélie Bertin ()
Additional contact information
Alexandre Beber: CNRS UMR168
Cyntia Taveneau: CNRS UMR168
Manuela Nania: Imperial College London
Feng-Ching Tsai: CNRS UMR168
Aurelie Cicco: CNRS UMR168
Patricia Bassereau: CNRS UMR168
Daniel Lévy: CNRS UMR168
João T. Cabral: Imperial College London
Hervé Isambert: CNRS UMR168
Stéphanie Mangenot: CNRS UMR168
Aurélie Bertin: CNRS UMR168

Nature Communications, 2019, vol. 10, issue 1, 1-12

Abstract: Abstract Septins are cytoskeletal filaments that assemble at the inner face of the plasma membrane. They are localized at constriction sites and impact membrane remodeling. We report in vitro tools to examine how yeast septins behave on curved and deformable membranes. Septins reshape the membranes of Giant Unilamellar Vesicles with the formation of periodic spikes, while flattening smaller vesicles. We show that membrane deformations are associated to preferential arrangement of septin filaments on specific curvatures. When binding to bilayers supported on custom-designed periodic wavy patterns displaying positive and negative micrometric radii of curvatures, septin filaments remain straight and perpendicular to the curvature of the convex parts, while bending negatively to follow concave geometries. Based on these results, we propose a theoretical model that describes the deformations and micrometric curvature sensitivity observed in vitro. The model captures the reorganizations of septin filaments throughout cytokinesis in vivo, providing mechanistic insights into cell division.

Date: 2019
References: Add references at CitEc
Citations: View citations in EconPapers (1)

Downloads: (external link)
https://www.nature.com/articles/s41467-019-08344-5 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-08344-5

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-019-08344-5

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().