Dual RNA-seq identifies human mucosal immunity protein Mucin-13 as a hallmark of Plasmodium exoerythrocytic infection

Gregory M. LaMonte, Pamela Orjuela-Sanchez, Jaeson Calla, Lawrence T. Wang, Shangzhong Li, Justine Swann, Annie N. Cowell, Bing Yu Zou, Alyaa M. Abdel-Haleem Mohamed, Zaira Hellen Villa Galarce, Marta Moreno, Carlos Tong Rios, Joseph M. Vinetz, Nathan Lewis and Elizabeth A. Winzeler ()
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Gregory M. LaMonte: University of California, San Diego, School of Medicine
Pamela Orjuela-Sanchez: University of California, San Diego, School of Medicine
Jaeson Calla: University of California, San Diego, School of Medicine
Lawrence T. Wang: University of California, San Diego, School of Medicine
Shangzhong Li: University of California, San Diego, School of Medicine
Justine Swann: University of California, San Diego, School of Medicine
Annie N. Cowell: University of California San Diego
Bing Yu Zou: University of California, San Diego, School of Medicine
Alyaa M. Abdel-Haleem Mohamed: King Abdullah University of Science and Technology (KAUST)
Zaira Hellen Villa Galarce: Universidad Peruana Cayetano Heredia
Marta Moreno: Universidad Peruana Cayetano Heredia
Carlos Tong Rios: Universidad Peruana Cayetano Heredia
Joseph M. Vinetz: University of California San Diego
Nathan Lewis: University of California, San Diego, School of Medicine
Elizabeth A. Winzeler: University of California, San Diego, School of Medicine

Nature Communications, 2019, vol. 10, issue 1, 1-13

Abstract: Abstract The exoerythrocytic stage of Plasmodium infection is a critical window for prophylactic intervention. Using genome-wide dual RNA sequencing of flow-sorted infected and uninfected hepatoma cells we show that the human mucosal immunity gene, mucin-13 (MUC13), is strongly upregulated during Plasmodium exoerythrocytic hepatic-stage infection. We confirm MUC13 transcript increases in hepatoma cell lines and primary hepatocytes. In immunofluorescence assays, host MUC13 protein expression distinguishes infected cells from adjacent uninfected cells and shows similar colocalization with parasite biomarkers such as UIS4 and HSP70. We further show that localization patterns are species independent, marking both P. berghei and P. vivax infected cells, and that MUC13 can be used to identify compounds that inhibit parasite replication in hepatocytes. This data provides insights into host-parasite interactions in Plasmodium infection, and demonstrates that a component of host mucosal immunity is reprogrammed during the progression of infection.

Date: 2019
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DOI: 10.1038/s41467-019-08349-0

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