Structural basis of ECF-σ-factor-dependent transcription initiation

Wei Lin, Sukhendu Mandal, David Degen, Min Sung Cho, Yu Feng, Kalyan Das and Richard H. Ebright ()
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Wei Lin: Rutgers University
Sukhendu Mandal: Rutgers University
David Degen: Rutgers University
Min Sung Cho: Rutgers University
Yu Feng: Rutgers University
Kalyan Das: KU Leuven
Richard H. Ebright: Rutgers University

Nature Communications, 2019, vol. 10, issue 1, 1-14

Abstract: Abstract Extracytoplasmic (ECF) σ factors, the largest class of alternative σ factors, are related to primary σ factors, but have simpler structures, comprising only two of six conserved functional modules in primary σ factors: region 2 (σR2) and region 4 (σR4). Here, we report crystal structures of transcription initiation complexes containing Mycobacterium tuberculosis RNA polymerase (RNAP), M. tuberculosis ECF σ factor σL, and promoter DNA. The structures show that σR2 and σR4 of the ECF σ factor occupy the same sites on RNAP as in primary σ factors, show that the connector between σR2 and σR4 of the ECF σ factor–although shorter and unrelated in sequence–follows the same path through RNAP as in primary σ factors, and show that the ECF σ factor uses the same strategy to bind and unwind promoter DNA as primary σ factors. The results define protein-protein and protein-DNA interactions involved in ECF-σ-factor-dependent transcription initiation.

Date: 2019
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DOI: 10.1038/s41467-019-08443-3

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