A cell cycle-coordinated Polymerase II transcription compartment encompasses gene expression before global genome activation

Yavor Hadzhiev, Haseeb K. Qureshi, Lucy Wheatley, Ledean Cooper, Aleksandra Jasiulewicz, Huy Nguyen, Joseph W. Wragg, Divyasree Poovathumkadavil, Sascha Conic, Sarah Bajan, Attila Sik, György Hutvàgner, Làszlò Tora, Agnieszka Gambus, John S. Fossey and Ferenc Müller ()
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Yavor Hadzhiev: University of Birmingham
Haseeb K. Qureshi: University of Birmingham
Lucy Wheatley: University of Birmingham
Ledean Cooper: University of Birmingham
Aleksandra Jasiulewicz: University of Birmingham
Huy Nguyen: University of Birmingham
Joseph W. Wragg: University of Birmingham
Divyasree Poovathumkadavil: University of Birmingham
Sascha Conic: Centre National de la Recherche Scientifique
Sarah Bajan: University of Technology
Attila Sik: University of Pecs
György Hutvàgner: University of Technology
Làszlò Tora: Centre National de la Recherche Scientifique
Agnieszka Gambus: University of Birmingham
John S. Fossey: University of Birmingham
Ferenc Müller: University of Birmingham

Nature Communications, 2019, vol. 10, issue 1, 1-14

Abstract: Abstract Most metazoan embryos commence development with rapid, transcriptionally silent cell divisions, with genome activation delayed until the mid-blastula transition (MBT). However, a set of genes escapes global repression and gets activated before MBT. Here we describe the formation and the spatio-temporal dynamics of a pair of distinct transcription compartments, which encompasses the earliest gene expression in zebrafish. 4D imaging of pri-miR430 and zinc-finger-gene activities by a novel, native transcription imaging approach reveals transcriptional sharing of nuclear compartments, which are regulated by homologous chromosome organisation. These compartments carry the majority of nascent-RNAs and active Polymerase II, are chromatin-depleted and represent the main sites of detectable transcription before MBT. Transcription occurs during the S-phase of increasingly permissive cleavage cycles. It is proposed, that the transcription compartment is part of the regulatory architecture of embryonic nuclei and offers a transcriptionally competent environment to facilitate early escape from repression before global genome activation.

Date: 2019
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DOI: 10.1038/s41467-019-08487-5

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