Differential organization of tonic and chronic B cell antigen receptors in the plasma membrane

Maria Angela Gomes de Castro, Hanna Wildhagen, Shama Sograte-Idrissi, Christoffer Hitzing, Mascha Binder, Martin Trepel, Niklas Engels () and Felipe Opazo ()
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Maria Angela Gomes de Castro: University Medical Center Göttingen
Hanna Wildhagen: University Medical Center Göttingen
Shama Sograte-Idrissi: University Medical Center Göttingen
Christoffer Hitzing: University Medical Center Göttingen
Mascha Binder: University Medical Center Hamburg-Eppendorf
Martin Trepel: University Medical Center Hamburg-Eppendorf
Niklas Engels: University Medical Center Göttingen
Felipe Opazo: University Medical Center Göttingen

Nature Communications, 2019, vol. 10, issue 1, 1-11

Abstract: Abstract Stimulation of the B cell antigen receptor (BCR) triggers signaling pathways that promote the differentiation of B cells into plasma cells. Despite the pivotal function of BCR in B cell activation, the organization of the BCR on the surface of resting and antigen-activated B cells remains unclear. Here we show, using STED super-resolution microscopy, that IgM-containing BCRs exist predominantly as monomers and dimers in the plasma membrane of resting B cells, but form higher oligomeric clusters upon stimulation. By contrast, a chronic lymphocytic leukemia-derived BCR forms dimers and oligomers in the absence of a stimulus, but a single amino acid exchange reverts its organization to monomers in unstimulated B cells. Our super-resolution microscopy approach for quantitatively analyzing cell surface proteins may thus help reveal the nanoscale organization of immunoreceptors in various cell types.

Date: 2019
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DOI: 10.1038/s41467-019-08677-1

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