A three-dimensional ratiometric sensing strategy on unimolecular fluorescence–thermally activated delayed fluorescence dual emission

Xuping Li, Gleb Baryshnikov, Chao Deng, Xiaoyan Bao, Bin Wu, Yunyun Zhou, Hans Ågren and Liangliang Zhu ()
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Xuping Li: Fudan University
Gleb Baryshnikov: KTH Royal Institute of Technology
Chao Deng: Zhejiang University
Xiaoyan Bao: Fudan University
Bin Wu: Fudan University
Yunyun Zhou: Fudan University
Hans Ågren: KTH Royal Institute of Technology
Liangliang Zhu: Fudan University

Nature Communications, 2019, vol. 10, issue 1, 1-9

Abstract: Abstract Visualized sensing through fluorescence signals is a powerful method for chemical and physical detection. However, the utilization of fluorescent molecular probes still suffers from lack of precise signal self-calibration in practical use. Here we show that fluorescence and thermally activated delayed fluorescence can be simultaneously produced at the single-molecular level. The thermally activated delayed fluorescence serves as a sensing signal with its wavelength and lifetime both altered correlating to polarity, whereas the fluorescence always remains unchanged as an internal reference. Upon the establishment of a three-dimensional working curve upon the ratiometric wavelength and photoluminescence lifetime vs. polarity, disturbance factors during a relevant sensing process can be largely minimized by such a multiple self-calibration. This strategy was further applied into a precise detection of the microenvironmental polarity variation in complex phospholipid systems, towards providing new insights for convenient and accurate diagnosis of membrane lesions.

Date: 2019
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DOI: 10.1038/s41467-019-08684-2

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