The human gut Firmicute Roseburia intestinalis is a primary degrader of dietary β-mannans

Sabina Leanti La Rosa, Maria Louise Leth, Leszek Michalak, Morten Ejby Hansen, Nicholas A. Pudlo, Robert Glowacki, Gabriel Pereira, Christopher T. Workman, Magnus Ø. Arntzen, Phillip B. Pope, Eric C. Martens, Maher Abou Hachem and Bjørge Westereng ()
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Sabina Leanti La Rosa: Norwegian University of Life Sciences
Maria Louise Leth: Danish Technical University
Leszek Michalak: Norwegian University of Life Sciences
Morten Ejby Hansen: Danish Technical University
Nicholas A. Pudlo: University of Michigan Medical School
Robert Glowacki: University of Michigan Medical School
Gabriel Pereira: University of Michigan Medical School
Christopher T. Workman: Danish Technical University
Magnus Ø. Arntzen: Norwegian University of Life Sciences
Phillip B. Pope: Norwegian University of Life Sciences
Eric C. Martens: University of Michigan Medical School
Maher Abou Hachem: Danish Technical University
Bjørge Westereng: Norwegian University of Life Sciences

Nature Communications, 2019, vol. 10, issue 1, 1-14

Abstract: Abstract β-Mannans are plant cell wall polysaccharides that are commonly found in human diets. However, a mechanistic understanding into the key populations that degrade this glycan is absent, especially for the dominant Firmicutes phylum. Here, we show that the prominent butyrate-producing Firmicute Roseburia intestinalis expresses two loci conferring metabolism of β-mannans. We combine multi-“omic” analyses and detailed biochemical studies to comprehensively characterize loci-encoded proteins that are involved in β-mannan capturing, importation, de-branching and degradation into monosaccharides. In mixed cultures, R. intestinalis shares the available β-mannan with Bacteroides ovatus, demonstrating that the apparatus allows coexistence in a competitive environment. In murine experiments, β-mannan selectively promotes beneficial gut bacteria, exemplified by increased R. intestinalis, and reduction of mucus-degraders. Our findings highlight that R. intestinalis is a primary degrader of this dietary fiber and that this metabolic capacity could be exploited to selectively promote key members of the healthy microbiota using β-mannan-based therapeutic interventions.

Date: 2019
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DOI: 10.1038/s41467-019-08812-y

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