Protein and RNA dynamical fingerprinting

Katherine A. Niessen, Mengyang Xu, Deepu K. George, Michael C. Chen, Adrian R. Ferré-D’Amaré, Edward H. Snell, Vivian Cody, James Pace, Marius Schmidt and Andrea G. Markelz ()
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Katherine A. Niessen: University at Buffalo, SUNY
Mengyang Xu: University at Buffalo, SUNY
Deepu K. George: University at Buffalo, SUNY
Michael C. Chen: National Heart, Lung and Blood Institute
Adrian R. Ferré-D’Amaré: National Heart, Lung and Blood Institute
Edward H. Snell: University at Buffalo, SUNY
Vivian Cody: University at Buffalo, SUNY
James Pace: University at Buffalo, SUNY
Marius Schmidt: University of Wisconsin
Andrea G. Markelz: University at Buffalo, SUNY

Nature Communications, 2019, vol. 10, issue 1, 1-10

Abstract: Abstract Protein structural vibrations impact biology by steering the structure to functional intermediate states; enhancing tunneling events; and optimizing energy transfer. Strong water absorption and a broad continuous vibrational density of states have prevented optical identification of these vibrations. Recently spectroscopic signatures that change with functional state were measured using anisotropic terahertz microscopy. The technique however has complex sample positioning requirements and long measurement times, limiting access for the biomolecular community. Here we demonstrate that a simplified system increases spectroscopic structure to dynamically fingerprint biomacromolecules with a factor of 6 reduction in data acquisition time. Using this technique, polarization varying anisotropy terahertz microscopy, we show sensitivity to inhibitor binding and unique vibrational spectra for several proteins and an RNA G-quadruplex. The technique’s sensitivity to anisotropic absorbance and birefringence provides rapid assessment of macromolecular dynamics that impact biology.

Date: 2019
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DOI: 10.1038/s41467-019-08926-3

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