Cdk1-mediated DIAPH1 phosphorylation maintains metaphase cortical tension and inactivates the spindle assembly checkpoint at anaphase

Koutarou Nishimura, Yoshikazu Johmura, Katashi Deguchi, Zixian Jiang, Kazuhiko S. K. Uchida, Narumi Suzuki, Midori Shimada, Yoshie Chiba, Toru Hirota, Shige H. Yoshimura, Keiko Kono () and Makoto Nakanishi ()
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Koutarou Nishimura: Nagoya City University
Yoshikazu Johmura: Nagoya City University
Katashi Deguchi: Kyoto University
Zixian Jiang: Kyoto University
Kazuhiko S. K. Uchida: Cancer Institute of the Japanese Foundation for Cancer Research (JFCR)
Narumi Suzuki: The University of Tokyo
Midori Shimada: Yamaguchi University
Yoshie Chiba: The University of Tokyo
Toru Hirota: Cancer Institute of the Japanese Foundation for Cancer Research (JFCR)
Shige H. Yoshimura: Kyoto University
Keiko Kono: Nagoya City University
Makoto Nakanishi: Nagoya City University

Nature Communications, 2019, vol. 10, issue 1, 1-12

Abstract: Abstract Animal cells undergo rapid rounding during mitosis, ensuring proper chromosome segregation, during which an outward rounding force abruptly increases upon prometaphase entry and is maintained at a constant level during metaphase. Initial cortical tension is generated by the actomyosin system to which both myosin motors and actin network architecture contribute. However, how cortical tension is maintained and its physiological significance remain unknown. We demonstrate here that Cdk1-mediated phosphorylation of DIAPH1 stably maintains cortical tension after rounding and inactivates the spindle assembly checkpoint (SAC). Cdk1 phosphorylates DIAPH1, preventing profilin1 binding to maintain cortical tension. Mutation of DIAPH1 phosphorylation sites promotes cortical F-actin accumulation, increases cortical tension, and delays anaphase onset due to SAC activation. Measurement of the intra-kinetochore length suggests that Cdk1-mediated cortex relaxation is indispensable for kinetochore stretching. We thus uncovered a previously unknown mechanism by which Cdk1 coordinates cortical tension maintenance and SAC inactivation at anaphase onset.

Date: 2019
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DOI: 10.1038/s41467-019-08957-w

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