The Wolbachia mobilome in Culex pipiens includes a putative plasmid

Julie Reveillaud (), Sarah R. Bordenstein, Corinne Cruaud, Alon Shaiber, Özcan C. Esen, Mylène Weill, Patrick Makoundou, Karen Lolans, Andrea R. Watson, Ignace Rakotoarivony, Seth R. Bordenstein and A. Murat Eren ()
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Julie Reveillaud: University of Montpellier
Sarah R. Bordenstein: Vanderbilt University
Corinne Cruaud: Commissariat à l’Energie Atomique et aux Energies Alternatives (CEA), Institut de Biologie François Jacob, Genoscope
Alon Shaiber: University of Chicago
Özcan C. Esen: University of Chicago
Mylène Weill: UMR CNRS-IRD-EPHE-Université de Montpellier
Patrick Makoundou: UMR CNRS-IRD-EPHE-Université de Montpellier
Karen Lolans: University of Chicago
Andrea R. Watson: University of Chicago
Ignace Rakotoarivony: University of Montpellier
Seth R. Bordenstein: Vanderbilt University
A. Murat Eren: University of Chicago

Nature Communications, 2019, vol. 10, issue 1, 1-11

Abstract: Abstract Wolbachia is a genus of obligate intracellular bacteria found in nematodes and arthropods worldwide, including insect vectors that transmit dengue, West Nile, and Zika viruses. Wolbachia’s unique ability to alter host reproductive behavior through its temperate bacteriophage WO has enabled the development of new vector control strategies. However, our understanding of Wolbachia’s mobilome beyond its bacteriophages is incomplete. Here, we reconstruct near-complete Wolbachia genomes from individual ovary metagenomes of four wild Culex pipiens mosquitoes captured in France. In addition to viral genes missing from the Wolbachia reference genome, we identify a putative plasmid (pWCP), consisting of a 9.23-kbp circular element with 14 genes. We validate its presence in additional Culex pipiens mosquitoes using PCR, long-read sequencing, and screening of existing metagenomes. The discovery of this previously unrecognized extrachromosomal element opens additional possibilities for genetic manipulation of Wolbachia.

Date: 2019
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DOI: 10.1038/s41467-019-08973-w

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