Bioengineered bacterial vesicles as biological nano-heaters for optoacoustic imaging

Vipul Gujrati, Jaya Prakash, Jaber Malekzadeh-Najafabadi, Andre Stiel, Uwe Klemm, Gabriele Mettenleiter, Michaela Aichler, Axel Walch and Vasilis Ntziachristos ()
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Vipul Gujrati: Technische Universität München
Jaya Prakash: Institute of Biological and Medical Imaging, Helmholtz Zentrum München
Jaber Malekzadeh-Najafabadi: Technische Universität München
Andre Stiel: Institute of Biological and Medical Imaging, Helmholtz Zentrum München
Uwe Klemm: Institute of Biological and Medical Imaging, Helmholtz Zentrum München
Gabriele Mettenleiter: Helmholtz Zentrum München
Michaela Aichler: Helmholtz Zentrum München
Axel Walch: Helmholtz Zentrum München
Vasilis Ntziachristos: Technische Universität München

Nature Communications, 2019, vol. 10, issue 1, 1-10

Abstract: Abstract Advances in genetic engineering have enabled the use of bacterial outer membrane vesicles (OMVs) to deliver vaccines, drugs and immunotherapy agents, as a strategy to circumvent biocompatibility and large-scale production issues associated with synthetic nanomaterials. We investigate bioengineered OMVs for contrast enhancement in optoacoustic (photoacoustic) imaging. We produce OMVs encapsulating biopolymer-melanin (OMVMel) using a bacterial strain expressing a tyrosinase transgene. Our results show that upon near-infrared light irradiation, OMVMel generates strong optoacoustic signals appropriate for imaging applications. In addition, we show that OMVMel builds up intense heat from the absorbed laser energy and mediates photothermal effects both in vitro and in vivo. Using multispectral optoacoustic tomography, we noninvasively monitor the spatio-temporal, tumour-associated OMVMel distribution in vivo. This work points to the use of bioengineered vesicles as potent alternatives to synthetic particles more commonly employed for optoacoustic imaging, with the potential to enable both image enhancement and photothermal applications.

Date: 2019
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DOI: 10.1038/s41467-019-09034-y

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