Phototrophic purple bacteria as optoacoustic in vivo reporters of macrophage activity

Lena Peters, Ina Weidenfeld, Uwe Klemm, Anita Loeschcke, Robin Weihmann, Karl-Erich Jaeger, Thomas Drepper (), Vasilis Ntziachristos and Andre C. Stiel ()
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Lena Peters: Forschungszentrum Jülich GmbH
Ina Weidenfeld: Helmholtz Zentrum München
Uwe Klemm: Helmholtz Zentrum München
Anita Loeschcke: Forschungszentrum Jülich GmbH
Robin Weihmann: Forschungszentrum Jülich GmbH
Karl-Erich Jaeger: Forschungszentrum Jülich GmbH
Thomas Drepper: Forschungszentrum Jülich GmbH
Vasilis Ntziachristos: Helmholtz Zentrum München
Andre C. Stiel: Helmholtz Zentrum München

Nature Communications, 2019, vol. 10, issue 1, 1-9

Abstract: Abstract Τhe morphology, physiology and immunology, of solid tumors exhibit spatial heterogeneity which complicates our understanding of cancer progression and therapy response. Understanding spatial heterogeneity necessitates high resolution in vivo imaging of anatomical and pathophysiological tumor information. We introduce Rhodobacter as bacterial reporter for multispectral optoacoustic (photoacoustic) tomography (MSOT). We show that endogenous bacteriochlorophyll a in Rhodobacter gives rise to strong optoacoustic signals >800 nm away from interfering endogenous absorbers. Importantly, our results suggest that changes in the spectral signature of Rhodobacter which depend on macrophage activity inside the tumor can be used to reveal heterogeneity of the tumor microenvironment. Employing non-invasive high resolution MSOT in longitudinal studies we show spatiotemporal changes of Rhodobacter spectral profiles in mice bearing 4T1 and CT26.WT tumor models. Accessibility of Rhodobacter to genetic modification and thus to sensory and therapeutic functions suggests potential for a theranostic platform organism.

Date: 2019
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DOI: 10.1038/s41467-019-09081-5

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