Mesoscopic and microscopic imaging of sensory responses in the same animal

Davide Boido, Ravi L. Rungta, Bruno-Félix Osmanski, Morgane Roche, Tomokazu Tsurugizawa, Denis Bihan, Luisa Ciobanu and Serge Charpak ()
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Davide Boido: Université Paris Descartes
Ravi L. Rungta: Université Paris Descartes
Bruno-Félix Osmanski: Université Paris Descartes
Morgane Roche: Université Paris Descartes
Tomokazu Tsurugizawa: NeuroSpin, Bât 145, Commissariat à l’Energie Atomique-Saclay Center
Denis Bihan: NeuroSpin, Bât 145, Commissariat à l’Energie Atomique-Saclay Center
Luisa Ciobanu: NeuroSpin, Bât 145, Commissariat à l’Energie Atomique-Saclay Center
Serge Charpak: Université Paris Descartes

Nature Communications, 2019, vol. 10, issue 1, 1-13

Abstract: Abstract Imaging based on blood flow dynamics is widely used to study sensory processing. Here we investigated the extent to which local neuronal and capillary responses (two-photon microscopy) are correlated to mesoscopic responses detected with fast ultrasound (fUS) and BOLD-fMRI. Using a specialized chronic olfactory bulb preparation, we report that sequential imaging of the same mouse allows quantitative comparison of odour responses, imaged at both microscopic and mesoscopic scales. Under these conditions, functional hyperaemia occurred at the threshold of neuronal activation and fUS-CBV signals could be detected at the level of single voxels with activation maps varying according to blood velocity. Both neuronal and vascular responses increase non-linearly as a function of odour concentration, whereas both microscopic and mesoscopic vascular responses are linearly correlated to local neuronal calcium. These data establish strengths and limits of mesoscopic imaging techniques to report neural activity.

Date: 2019
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DOI: 10.1038/s41467-019-09082-4

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