Quantifying protein dynamics and stability in a living organism

Ruopei Feng, Martin Gruebele () and Caitlin M. Davis ()
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Ruopei Feng: University of Illinois at Urbana-Champaign
Martin Gruebele: University of Illinois at Urbana-Champaign
Caitlin M. Davis: University of Illinois at Urbana-Champaign

Nature Communications, 2019, vol. 10, issue 1, 1-7

Abstract: Abstract As an integral part of modern cell biology, fluorescence microscopy enables quantification of the stability and dynamics of fluorescence-labeled biomolecules inside cultured cells. However, obtaining time-resolved data from individual cells within a live vertebrate organism remains challenging. Here we demonstrate a customized pipeline that integrates meganuclease-mediated mosaic transformation with fluorescence-detected temperature-jump microscopy to probe dynamics and stability of endogenously expressed proteins in different tissues of living multicellular organisms.

Date: 2019
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DOI: 10.1038/s41467-019-09088-y

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