Membrane-cytoskeletal crosstalk mediated by myosin-I regulates adhesion turnover during phagocytosis

Sarah R. Barger, Nicholas S. Reilly, Maria S. Shutova, Qingsen Li, Paolo Maiuri, John M. Heddleston, Mark S. Mooseker, Richard A. Flavell, Tatyana Svitkina, Patrick W. Oakes, Mira Krendel () and Nils C. Gauthier ()
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Sarah R. Barger: State University of New York Upstate Medical University
Nicholas S. Reilly: University of Rochester
Maria S. Shutova: University of Pennsylvania
Qingsen Li: FIRC Institute of Molecular Oncology
Paolo Maiuri: FIRC Institute of Molecular Oncology
John M. Heddleston: Howard Hughes Medical Institute Janelia Research Campus
Mark S. Mooseker: Yale University
Richard A. Flavell: Yale University School of Medicine
Tatyana Svitkina: University of Pennsylvania
Patrick W. Oakes: University of Rochester
Mira Krendel: State University of New York Upstate Medical University
Nils C. Gauthier: FIRC Institute of Molecular Oncology

Nature Communications, 2019, vol. 10, issue 1, 1-18

Abstract: Abstract Phagocytosis of invading pathogens or cellular debris requires a dramatic change in cell shape driven by actin polymerization. For antibody-covered targets, phagocytosis is thought to proceed through the sequential engagement of Fc-receptors on the phagocyte with antibodies on the target surface, leading to the extension and closure of the phagocytic cup around the target. We find that two actin-dependent molecular motors, class 1 myosins myosin 1e and myosin 1f, are specifically localized to Fc-receptor adhesions and required for efficient phagocytosis of antibody-opsonized targets. Using primary macrophages lacking both myosin 1e and myosin 1f, we find that without the actin-membrane linkage mediated by these myosins, the organization of individual adhesions is compromised, leading to excessive actin polymerization, slower adhesion turnover, and deficient phagocytic internalization. This work identifies a role for class 1 myosins in coordinated adhesion turnover during phagocytosis and supports a mechanism involving membrane-cytoskeletal crosstalk for phagocytic cup closure.

Date: 2019
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DOI: 10.1038/s41467-019-09104-1

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