Waveguide-PAINT offers an open platform for large field-of-view super-resolution imaging

Anna Archetti, Evgenii Glushkov, Christian Sieben, Anton Stroganov, Aleksandra Radenovic and Suliana Manley ()
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Anna Archetti: Institutes of Physics and Bioengineering, École Polytechnique Fédérale de Lausanne (EPFL)
Evgenii Glushkov: Institute of Bioengineering, École Polytechnique Fédérale de Lausanne (EPFL)
Christian Sieben: Institutes of Physics and Bioengineering, École Polytechnique Fédérale de Lausanne (EPFL)
Anton Stroganov: Institutes of Physics and Bioengineering, École Polytechnique Fédérale de Lausanne (EPFL)
Aleksandra Radenovic: Institute of Bioengineering, École Polytechnique Fédérale de Lausanne (EPFL)
Suliana Manley: Institutes of Physics and Bioengineering, École Polytechnique Fédérale de Lausanne (EPFL)

Nature Communications, 2019, vol. 10, issue 1, 1-9

Abstract: Abstract Super-resolution microscopies based on the localization of single molecules have been widely adopted due to their demonstrated performance and their accessibility resulting from open software and simple hardware. The PAINT method for localization microscopy offers improved resolution over photoswitching methods, since it is less prone to sparse sampling of structures and provides higher localization precision. Here, we show that waveguides enable increased throughput and data quality for PAINT, by generating a highly uniform ~100 × 2000 µm2 area evanescent field for TIRF illumination. To achieve this, we designed and fabricated waveguides optimized for efficient light coupling and propagation, incorporating a carefully engineered input facet and taper. We also developed a stable, low-cost microscope and 3D-printable waveguide chip holder for easy alignment and imaging. We demonstrate the capabilities of our open platform by using DNA-PAINT to image multiple whole cells or hundreds of origami structures in a single field of view.

Date: 2019
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DOI: 10.1038/s41467-019-09247-1

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