Assessing the viability of transplanted gut microbiota by sequential tagging with D-amino acid-based metabolic probes

Wei Wang, Liyuan Lin, Yahui Du, Yanling Song, Xiaoman Peng, Xing Chen () and Chaoyong James Yang ()
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Wei Wang: Shanghai Jiao Tong University School of Medicine
Liyuan Lin: Shanghai Jiao Tong University School of Medicine
Yahui Du: Xiamen University
Yanling Song: Shanghai Jiao Tong University School of Medicine
Xiaoman Peng: Shanghai Jiao Tong University School of Medicine
Xing Chen: Peking University
Chaoyong James Yang: Shanghai Jiao Tong University School of Medicine

Nature Communications, 2019, vol. 10, issue 1, 1-7

Abstract: Abstract Currently, there are more than 200 fecal microbiota transplantation (FMT) clinical trials worldwide. However, our knowledge of this microbial therapy is still limited. Here we develop a strategy using sequential tagging with D-amino acid-based metabolic probes (STAMP) for assessing the viabilities of transplanted microbiotas. A fluorescent D-amino acid (FDAA) is first administered to donor mice to metabolically label the gut microbiotas in vivo. The labeled microbiotas are transplanted to recipient mice, which receive a second FDAA with a different color. The surviving transplants should incorporate both FDAAs and can be readily distinguished by presenting two colors simultaneously. Isolation of surviving bacteria and 16S rDNA sequencing identify several enriched genera, suggesting the importance of specific bacteria in FMT. In addition, using STAMP, we evaluate the effects on transplant survival of pre-treating recipients using different antibiotics. We propose STAMP as a versatile tool for deciphering the complex biology of FMT, and potentially improving its treatment efficacy.

Date: 2019
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DOI: 10.1038/s41467-019-09267-x

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