Mad1 destabilizes p53 by preventing PML from sequestering MDM2

Jun Wan, Samuel Block, Christina M. Scribano, Rebecca Thiry, Karla Esbona, Anjon Audhya and Beth A. Weaver ()
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Jun Wan: University of Wisconsin-Madison
Samuel Block: University of Wisconsin-Madison
Christina M. Scribano: University of Wisconsin-Madison
Rebecca Thiry: University of Wisconsin-Madison
Karla Esbona: University of Wisconsin-Madison
Anjon Audhya: University of Wisconsin-Madison
Beth A. Weaver: University of Wisconsin-Madison

Nature Communications, 2019, vol. 10, issue 1, 1-14

Abstract: Abstract Mitotic arrest deficient 1 (Mad1) plays a well-characterized role in the mitotic checkpoint. However, interphase roles of Mad1 that do not impact mitotic checkpoint function remain largely uncharacterized. Here we show that upregulation of Mad1, which is common in human breast cancer, prevents stress-induced stabilization of the tumor suppressor p53 in multiple cell types. Upregulated Mad1 localizes to ProMyelocytic Leukemia (PML) nuclear bodies in breast cancer and cultured cells. The C-terminus of Mad1 directly interacts with PML, and this interaction is enhanced by sumoylation. PML stabilizes p53 by sequestering MDM2, an E3 ubiquitin ligase that targets p53 for degradation, to the nucleolus. Upregulated Mad1 displaces MDM2 from PML, freeing it to ubiquitinate p53. Upregulation of Mad1 accelerates growth of orthotopic mammary tumors, which show decreased levels of p53 and its downstream effector p21. These results demonstrate an unexpected interphase role for Mad1 in tumor promotion via p53 destabilization.

Date: 2019
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-09471-9

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DOI: 10.1038/s41467-019-09471-9

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