High density DNA data storage library via dehydration with digital microfluidic retrieval

Sharon Newman, Ashley P. Stephenson, Max Willsey, Bichlien H. Nguyen, Christopher N. Takahashi, Karin Strauss and Luis Ceze ()
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Sharon Newman: University of Washington
Ashley P. Stephenson: University of Washington
Max Willsey: University of Washington
Bichlien H. Nguyen: University of Washington
Christopher N. Takahashi: University of Washington
Karin Strauss: University of Washington
Luis Ceze: University of Washington

Nature Communications, 2019, vol. 10, issue 1, 1-6

Abstract: Abstract DNA promises to be a high density data storage medium, but physical storage poses a challenge. To store large amounts of data, pools must be physically isolated so they can share the same addressing scheme. We propose the storage of dehydrated DNA spots on glass as an approach for scalable DNA data storage. The dried spots can then be retrieved by a water droplet using a digital microfluidic device. Here we show that this storage schema works with varying spot organization, spotted masses of DNA, and droplet retrieval dwell times. In all cases, the majority of the DNA was retrieved and successfully sequenced. We demonstrate that the spots can be densely arranged on a microfluidic device without significant contamination of the retrieval. We also demonstrate that 1 TB of data could be stored in a single spot of DNA and successfully retrieved using this method.

Date: 2019
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DOI: 10.1038/s41467-019-09517-y

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