Influenza A virus ribonucleoproteins form liquid organelles at endoplasmic reticulum exit sites

Marta Alenquer, Sílvia Vale-Costa, Temitope Akhigbe Etibor, Filipe Ferreira, Ana Laura Sousa and Maria João Amorim ()
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Marta Alenquer: Instituto Gulbenkian de Ciência
Sílvia Vale-Costa: Instituto Gulbenkian de Ciência
Temitope Akhigbe Etibor: Instituto Gulbenkian de Ciência
Filipe Ferreira: Instituto Gulbenkian de Ciência
Ana Laura Sousa: Instituto Gulbenkian de Ciência
Maria João Amorim: Instituto Gulbenkian de Ciência

Nature Communications, 2019, vol. 10, issue 1, 1-19

Abstract: Abstract Influenza A virus has an eight-partite RNA genome that during viral assembly forms a complex containing one copy of each RNA. Genome assembly is a selective process driven by RNA-RNA interactions and is hypothesized to lead to discrete punctate structures scattered through the cytosol. Here, we show that contrary to the accepted view, formation of these structures precedes RNA-RNA interactions among distinct viral ribonucleoproteins (vRNPs), as they assemble in cells expressing only one vRNP type. We demonstrate that these viral inclusions display characteristics of liquid organelles, segregating from the cytosol without a delimitating membrane, dynamically exchanging material and adapting fast to environmental changes. We provide evidence that viral inclusions develop close to endoplasmic reticulum (ER) exit sites, depend on continuous ER-Golgi vesicular cycling and do not promote escape to interferon response. We propose that viral inclusions segregate vRNPs from the cytosol and facilitate selected RNA-RNA interactions in a liquid environment.

Date: 2019
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DOI: 10.1038/s41467-019-09549-4

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