Ca2+-dependent regulation of sodium channels NaV1.4 and NaV1.5 is controlled by the post-IQ motif

Jesse B. Yoder, Manu Ben-Johny, Federica Farinelli, Lakshmi Srinivasan, Sophie R. Shoemaker, Gordon F. Tomaselli, Sandra B. Gabelli () and L. Mario Amzel ()
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Jesse B. Yoder: Johns Hopkins University School of Medicine
Manu Ben-Johny: Columbia University
Federica Farinelli: Johns Hopkins University School of Medicine
Lakshmi Srinivasan: Johns Hopkins University School of Medicine
Sophie R. Shoemaker: Johns Hopkins University School of Medicine
Gordon F. Tomaselli: Albert Einstein College of Medicine
Sandra B. Gabelli: Johns Hopkins University School of Medicine
L. Mario Amzel: Johns Hopkins University School of Medicine

Nature Communications, 2019, vol. 10, issue 1, 1-12

Abstract: Abstract Skeletal muscle voltage-gated Na+ channel (NaV1.4) activity is subject to calmodulin (CaM) mediated Ca2+-dependent inactivation; no such inactivation is observed in the cardiac Na+ channel (NaV1.5). Taken together, the crystal structures of the NaV1.4 C-terminal domain relevant complexes and thermodynamic binding data presented here provide a rationale for this isoform difference. A Ca2+-dependent CaM N-lobe binding site previously identified in NaV1.5 is not present in NaV1.4 allowing the N-lobe to signal other regions of the NaV1.4 channel. Consistent with this mechanism, removing this binding site in NaV1.5 unveils robust Ca2+-dependent inactivation in the previously insensitive isoform. These findings suggest that Ca2+-dependent inactivation is effected by CaM’s N-lobe binding outside the NaV C-terminal while CaM’s C-lobe remains bound to the NaV C-terminal. As the N-lobe binding motif of NaV1.5 is a mutational hotspot for inherited arrhythmias, the contributions of mutation-induced changes in CDI to arrhythmia generation is an intriguing possibility.

Date: 2019
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DOI: 10.1038/s41467-019-09570-7

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