iFISH is a publically available resource enabling versatile DNA FISH to study genome architecture

Eleni Gelali, Gabriele Girelli, Masahiro Matsumoto, Erik Wernersson, Joaquin Custodio, Ana Mota, Maud Schweitzer, Katalin Ferenc, Xinge Li, Reza Mirzazadeh, Federico Agostini, John P. Schell, Fredrik Lanner, Nicola Crosetto () and Magda Bienko ()
Additional contact information
Eleni Gelali: Karolinska Institutet
Gabriele Girelli: Karolinska Institutet
Masahiro Matsumoto: Medical Business Group, Sony Imaging Products & Solutions, Inc.
Erik Wernersson: Karolinska Institutet
Joaquin Custodio: Karolinska Institutet
Ana Mota: Karolinska Institutet
Maud Schweitzer: Karolinska Institutet
Katalin Ferenc: Karolinska Institutet
Xinge Li: Karolinska Institutet
Reza Mirzazadeh: Karolinska Institutet
Federico Agostini: Karolinska Institutet
John P. Schell: Karolinska Institutet
Fredrik Lanner: Karolinska Institutet
Nicola Crosetto: Karolinska Institutet
Magda Bienko: Karolinska Institutet

Nature Communications, 2019, vol. 10, issue 1, 1-15

Abstract: Abstract DNA fluorescence in situ hybridization (DNA FISH) is a powerful method to study chromosomal organization in single cells. At present, there is a lack of free resources of DNA FISH probes and probe design tools which can be readily applied. Here, we describe iFISH, an open-source repository currently comprising 380 DNA FISH probes targeting multiple loci on the human autosomes and chromosome X, as well as a genome-wide database of optimally designed oligonucleotides and a freely accessible web interface ( http://ifish4u.org ) that can be used to design DNA FISH probes. We individually validate 153 probes and take advantage of our probe repository to quantify the extent of intermingling between multiple heterologous chromosome pairs, showing a much higher extent of intermingling in human embryonic stem cells compared to fibroblasts. In conclusion, iFISH is a versatile and expandable resource, which can greatly facilitate the use of DNA FISH in research and diagnostics.

Date: 2019
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DOI: 10.1038/s41467-019-09616-w

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