KIBRA controls exosome secretion via inhibiting the proteasomal degradation of Rab27a

Lin Song, Shi Tang, Xiaolei Han, Ziying Jiang, Lingling Dong, Cuicui Liu, Xiaoyan Liang, Jixin Dong, Chengxuan Qiu, Yongxiang Wang () and Yifeng Du ()
Additional contact information
Lin Song: Shandong Provincial Hospital affiliated to Shandong University
Shi Tang: Shandong Provincial Hospital affiliated to Shandong University
Xiaolei Han: Shandong Provincial Hospital affiliated to Shandong University
Ziying Jiang: Shandong Provincial Hospital affiliated to Shandong University
Lingling Dong: Dongying People’s Hospital
Cuicui Liu: Shandong Provincial Hospital affiliated to Shandong University
Xiaoyan Liang: Shandong Provincial Hospital affiliated to Shandong University
Jixin Dong: University of Nebraska Medical Center
Chengxuan Qiu: Shandong Provincial Hospital affiliated to Shandong University
Yongxiang Wang: Shandong Provincial Hospital affiliated to Shandong University
Yifeng Du: Shandong Provincial Hospital affiliated to Shandong University

Nature Communications, 2019, vol. 10, issue 1, 1-13

Abstract: Abstract Exosomes are nanosized membrane vesicles released from cells after fusion of multivesicular bodies (MVBs) with the plasma membrane (PM) and play important roles in intercellular communication and numerous biological processes. However, the molecular mechanisms regulating exosome secretion remain poorly understood. Here we identify KIBRA as an adaptor-like protein that stabilizes Rab27a, which in turn controls exosome secretion both in vitro and in vivo. Knockdown or overexpression of KIBRA in neuronal and podocyte cell lines leads to a decrease or increase of exosome secretion, respectively, and KIBRA depletion increases MVB size and number. Comparing protein profiles between KIBRA knockout and wild-type mouse brain showed significantly decreased Rab27a, a small GTPase that regulates MVB-PM docking. Rab27a is stabilized by interacting with KIBRA, which prevents ubiquitination and degradation via the ubiquitin-proteasome pathway. In conclusion, we show that KIBRA controls exosome secretion via inhibiting the proteasomal degradation of Rab27a.

Date: 2019
References: Add references at CitEc
Citations: View citations in EconPapers (1)

Downloads: (external link)
https://www.nature.com/articles/s41467-019-09720-x Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-09720-x

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-019-09720-x

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().