Immunoregulation of macrophages by dynamic ligand presentation via ligand–cation coordination

Heemin Kang, Boguang Yang, Kunyu Zhang, Qi Pan, Weihao Yuan, Gang Li and Liming Bian ()
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Heemin Kang: The Chinese University of Hong Kong
Boguang Yang: The Chinese University of Hong Kong
Kunyu Zhang: The Chinese University of Hong Kong
Qi Pan: The Chinese University of Hong Kong, Prince of Wales Hospital
Weihao Yuan: The Chinese University of Hong Kong
Gang Li: The Chinese University of Hong Kong, Prince of Wales Hospital
Liming Bian: The Chinese University of Hong Kong

Nature Communications, 2019, vol. 10, issue 1, 1-14

Abstract: Abstract Macrophages regulate host responses to implants through their dynamic adhesion, release, and activation. Herein, we employ bisphosphonate (BP)-coated gold nanoparticle template (BNP) to direct the swift and convertible formation of Mg2+-functional Mg2+-BP nanoparticle (NP) on the BP-AuNP surface via reversible Mg2+-BP coordination, thus producing (Mg2+-BP)-Au dimer (MgBNP). Ethylenediaminetetraacetic acid-based Mg2+ chelation facilitates the dissolution of Mg2+-BP NP, thus enabling the reversion of the MgBNP to the BNP. This convertible nanoassembly incorporating cell-adhesive Mg2+ moieties directs reversible attachment and detachment of macrophages by BP and EDTA, without physical scraping or trypsin that could damage cells. The swift formation of RGD ligand- and Mg2+-bifunctional RGD-Mg2+-BP NP that yields (RGD-Mg2+-BP)-Au dimer (RGDBNP) further stimulates the adhesion and pro-regenerative M2-type polarization of macrophages, both in vitro and in vivo, including rho-associated protein kinase. This swift and non-toxic dimer formation can include diverse bio-functional moieties to regulate host responses to implants.

Date: 2019
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DOI: 10.1038/s41467-019-09733-6

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