Identification of peripheral neural circuits that regulate heart rate using optogenetic and viral vector strategies

Pradeep S. Rajendran, Rosemary C. Challis, Charless C. Fowlkes, Peter Hanna, John D. Tompkins, Maria C. Jordan, Sarah Hiyari, Beth A. Gabris-Weber, Alon Greenbaum, Ken Y. Chan, Benjamin E. Deverman, Heike Münzberg, Jeffrey L. Ardell, Guy Salama, Viviana Gradinaru () and Kalyanam Shivkumar ()
Additional contact information
Pradeep S. Rajendran: University of California - Los Angeles (UCLA)
Rosemary C. Challis: California Institute of Technology
Charless C. Fowlkes: University of California - Irvine
Peter Hanna: University of California - Los Angeles (UCLA)
John D. Tompkins: University of California - Los Angeles (UCLA)
Maria C. Jordan: University of California - Los Angeles (UCLA)
Sarah Hiyari: University of California - Los Angeles (UCLA)
Beth A. Gabris-Weber: University of Pittsburgh
Alon Greenbaum: California Institute of Technology
Ken Y. Chan: California Institute of Technology
Benjamin E. Deverman: California Institute of Technology
Heike Münzberg: Louisiana State University
Jeffrey L. Ardell: University of California - Los Angeles (UCLA)
Guy Salama: University of Pittsburgh
Viviana Gradinaru: California Institute of Technology
Kalyanam Shivkumar: University of California - Los Angeles (UCLA)

Nature Communications, 2019, vol. 10, issue 1, 1-13

Abstract: Abstract Heart rate is under the precise control of the autonomic nervous system. However, the wiring of peripheral neural circuits that regulate heart rate is poorly understood. Here, we develop a clearing-imaging-analysis pipeline to visualize innervation of intact hearts in 3D and employed a multi-technique approach to map parasympathetic and sympathetic neural circuits that control heart rate in mice. We identify cholinergic neurons and noradrenergic neurons in an intrinsic cardiac ganglion and the stellate ganglia, respectively, that project to the sinoatrial node. We also report that the heart rate response to optogenetic versus electrical stimulation of the vagus nerve displays different temporal characteristics and that vagal afferents enhance parasympathetic and reduce sympathetic tone to the heart via central mechanisms. Our findings provide new insights into neural regulation of heart rate, and our methodology to study cardiac circuits can be readily used to interrogate neural control of other visceral organs.

Date: 2019
References: Add references at CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/s41467-019-09770-1 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-09770-1

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-019-09770-1

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().