Highly sensitive CE-ESI-MS analysis of N-glycans from complex biological samples

Guinevere S. M. Lageveen-Kammeijer (), Noortje Haan, Pablo Mohaupt, Sander Wagt, Mike Filius, Jan Nouta, David Falck and Manfred Wuhrer
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Guinevere S. M. Lageveen-Kammeijer: Center for Proteomics and Metabolomics
Noortje Haan: Center for Proteomics and Metabolomics
Pablo Mohaupt: Center for Proteomics and Metabolomics
Sander Wagt: Center for Proteomics and Metabolomics
Mike Filius: Center for Proteomics and Metabolomics
Jan Nouta: Center for Proteomics and Metabolomics
David Falck: Center for Proteomics and Metabolomics
Manfred Wuhrer: Center for Proteomics and Metabolomics

Nature Communications, 2019, vol. 10, issue 1, 1-8

Abstract: Abstract The in-depth, high-sensitivity characterization of the glycome from complex biological samples, such as biofluids and tissues, is of utmost importance in basic biological research and biomarker discovery. Major challenges often arise from the vast structural diversity of glycans in combination with limited sample amounts. Here, we present a method for the highly sensitive characterization of released N-glycans by combining a capillary electrophoresis-electrospray ionization-mass spectrometry (CE-ESI-MS) approach with linkage-specific derivatization of sialic acids and uniform cationic reducing end labelling of all glycans. This method allows the analysis of glycans at the attomole level, provides information on sialic acid isomers and enables the in-depth characterization of complex samples, even when available in minute amounts.

Date: 2019
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-09910-7

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DOI: 10.1038/s41467-019-09910-7

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