Lipopolysaccharide inhalation recruits monocytes and dendritic cell subsets to the alveolar airspace

Laura Jardine (), Sarah Wiscombe, Gary Reynolds, David McDonald, Andrew Fuller, Kile Green, Andrew Filby, Ian Forrest, Marie-Helene Ruchaud-Sparagano, Jonathan Scott, Matthew Collin, Muzlifah Haniffa () and A. John Simpson
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Laura Jardine: Newcastle University
Sarah Wiscombe: Newcastle University
Gary Reynolds: Newcastle University
David McDonald: Newcastle University
Andrew Fuller: Newcastle University
Kile Green: Newcastle University
Andrew Filby: Newcastle University
Ian Forrest: Newcastle upon Tyne Hospitals NHS Foundation Trust
Marie-Helene Ruchaud-Sparagano: Newcastle University
Jonathan Scott: Newcastle University
Matthew Collin: Newcastle University
Muzlifah Haniffa: Newcastle University
A. John Simpson: Newcastle University

Nature Communications, 2019, vol. 10, issue 1, 1-13

Abstract: Abstract Mononuclear phagocytes (MPs) including monocytes, macrophages and dendritic cells (DCs) are critical innate immune effectors and initiators of the adaptive immune response. MPs are present in the alveolar airspace at steady state, however little is known about DC recruitment in acute pulmonary inflammation. Here we use lipopolysaccharide inhalation to induce acute inflammation in healthy volunteers and examine the impact on bronchoalveolar lavage fluid and blood MP repertoire. Classical monocytes and two DC subsets (DC2/3 and DC5) are expanded in bronchoalveolar lavage fluid 8 h after lipopolysaccharide inhalation. Surface phenotyping, gene expression profiling and parallel analysis of blood indicate recruited DCs are blood-derived. Recruited monocytes and DCs rapidly adopt typical airspace-resident MP gene expression profiles. Following lipopolysaccharide inhalation, alveolar macrophages strongly up-regulate cytokines for MP recruitment. Our study defines the characteristics of human DCs and monocytes recruited into bronchoalveolar space immediately following localised acute inflammatory stimulus in vivo.

Date: 2019
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DOI: 10.1038/s41467-019-09913-4

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