Differential human antibody repertoires following Zika infection and the implications for serodiagnostics and disease outcome

Supriya Ravichandran, Megan Hahn, Pablo F. Belaunzarán-Zamudio, José Ramos-Castañeda, Gabriel Nájera-Cancino, Sandra Caballero-Sosa, Karla R. Navarro-Fuentes, Guillermo Ruiz-Palacios, Hana Golding, John H. Beigel and Surender Khurana ()
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Supriya Ravichandran: Center for Biologics Evaluation and Research (CBER), FDA
Megan Hahn: Center for Biologics Evaluation and Research (CBER), FDA
Pablo F. Belaunzarán-Zamudio: Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán
José Ramos-Castañeda: Instituto Nacional de Salud Publica
Gabriel Nájera-Cancino: Hospital Regional de Alta Especialidad Ciudad Salud
Sandra Caballero-Sosa: Instituto de Seguridad y Servicios Sociales de los Trabajadores del Estado
Karla R. Navarro-Fuentes: Instituto Mexicano del Seguro Social
Guillermo Ruiz-Palacios: Comisión Coordinadora de los Institutos Nacionales de Salud y Hospitales de Alta Especialidad, Ministry of Health
Hana Golding: Center for Biologics Evaluation and Research (CBER), FDA
John H. Beigel: Frederick National Laboratory for Cancer Research
Surender Khurana: Center for Biologics Evaluation and Research (CBER), FDA

Nature Communications, 2019, vol. 10, issue 1, 1-14

Abstract: Abstract Zika virus (ZIKV) outbreak in Americas led to extensive efforts to develop vaccines and ZIKV-specific diagnostics. In the current study, we use whole genome phage display library spanning the entire ZIKV genome (ZIKV-GFPDL) for in-depth immune profiling of IgG and IgM antibody repertoires in serum and urine longitudinal samples from individuals acutely infected with ZIKV. We observe a very diverse IgM immune repertoire encompassing the entire ZIKV polyprotein on day 0 in both serum and urine. ZIKV-specific IgG antibodies increase 10-fold between day 0 and day 7 in serum, but not in urine; these are highly focused on prM/E, NS1 and NS2B. Differential antibody affinity maturation is observed against ZIKV structural E protein compared with nonstructural protein NS1. Serum antibody affinity to ZIKV-E protein inversely correlates with ZIKV disease symptoms. Our study provides insight into unlinked evolution of immune response to ZIKV infection and identified unique targets for ZIKV serodiagnostics.

Date: 2019
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DOI: 10.1038/s41467-019-09914-3

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