The extracellular matrix protects Bacillus subtilis colonies from Pseudomonas invasion and modulates plant co-colonization

Carlos Molina-Santiago, John R. Pearson, Yurena Navarro, María Victoria Berlanga-Clavero, Andrés Mauricio Caraballo-Rodriguez, Daniel Petras, María Luisa García-Martín, Gaelle Lamon, Birgit Haberstein, Francisco M. Cazorla, Antonio de Vicente, Antoine Loquet, Pieter C. Dorrestein and Diego Romero ()
Additional contact information
Carlos Molina-Santiago: Universidad de Málaga, Bulevar Louis Pasteur 31 (Campus Universitario de Teatinos)
John R. Pearson: Nano-imaging Unit, Andalusian Centre for Nanomedicine and Biotechnology, BIONAND
Yurena Navarro: Universidad de Málaga, Bulevar Louis Pasteur 31 (Campus Universitario de Teatinos)
María Victoria Berlanga-Clavero: Universidad de Málaga, Bulevar Louis Pasteur 31 (Campus Universitario de Teatinos)
Andrés Mauricio Caraballo-Rodriguez: University of California San Diego, Collaborative Mass Spectrometry Innovation Center
Daniel Petras: University of California San Diego, Collaborative Mass Spectrometry Innovation Center
María Luisa García-Martín: Nano-imaging Unit, Andalusian Centre for Nanomedicine and Biotechnology, BIONAND
Gaelle Lamon: CNRS, Université Bordeaux, Institut Européen de Chimie et Biologie
Birgit Haberstein: CNRS, Université Bordeaux, Institut Européen de Chimie et Biologie
Francisco M. Cazorla: Universidad de Málaga, Bulevar Louis Pasteur 31 (Campus Universitario de Teatinos)
Antonio de Vicente: Universidad de Málaga, Bulevar Louis Pasteur 31 (Campus Universitario de Teatinos)
Antoine Loquet: CNRS, Université Bordeaux, Institut Européen de Chimie et Biologie
Pieter C. Dorrestein: University of California San Diego, Collaborative Mass Spectrometry Innovation Center
Diego Romero: Universidad de Málaga, Bulevar Louis Pasteur 31 (Campus Universitario de Teatinos)

Nature Communications, 2019, vol. 10, issue 1, 1-15

Abstract: Abstract Bacteria of the genera Pseudomonas and Bacillus can promote plant growth and protect plants from pathogens. However, the interactions between these plant-beneficial bacteria are understudied. Here, we explore the interaction between Bacillus subtilis 3610 and Pseudomonas chlororaphis PCL1606. We show that the extracellular matrix protects B. subtilis colonies from infiltration by P. chlororaphis. The absence of extracellular matrix results in increased fluidity and loss of structure of the B. subtilis colony. The P. chlororaphis type VI secretion system (T6SS) is activated upon contact with B. subtilis cells, and stimulates B. subtilis sporulation. Furthermore, we find that B. subtilis sporulation observed prior to direct contact with P. chlororaphis is mediated by histidine kinases KinA and KinB. Finally, we demonstrate the importance of the extracellular matrix and the T6SS in modulating the coexistence of the two species on melon plant leaves and seeds.

Date: 2019
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DOI: 10.1038/s41467-019-09944-x

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