A protein complex required for polar growth of rhizobial infection threads

Cheng-Wu Liu, Andrew Breakspear, Nicola Stacey, Kim Findlay, Jin Nakashima, Karunakaran Ramakrishnan, Miaoxia Liu, Fang Xie, Gabriella Endre, Fernanda Carvalho-Niebel, Giles E. D. Oldroyd, Michael K. Udvardi, Joëlle Fournier () and Jeremy D. Murray ()
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Cheng-Wu Liu: Cell and Developmental Biology, John Innes Centre
Andrew Breakspear: Cell and Developmental Biology, John Innes Centre
Nicola Stacey: Cell and Developmental Biology, John Innes Centre
Kim Findlay: Cell and Developmental Biology, John Innes Centre
Jin Nakashima: Noble Research Institute
Karunakaran Ramakrishnan: Cell and Developmental Biology, John Innes Centre
Miaoxia Liu: Chinese Academy of Sciences
Fang Xie: Chinese Academy of Sciences
Gabriella Endre: Institute of Plant Biology, Biological Research Centre
Fernanda Carvalho-Niebel: LIPM, Université de Toulouse, INRA, CNRS
Giles E. D. Oldroyd: University of Cambridge
Michael K. Udvardi: Noble Research Institute
Joëlle Fournier: LIPM, Université de Toulouse, INRA, CNRS
Jeremy D. Murray: Cell and Developmental Biology, John Innes Centre

Nature Communications, 2019, vol. 10, issue 1, 1-17

Abstract: Abstract During root nodule symbiosis, intracellular accommodation of rhizobia by legumes is a prerequisite for nitrogen fixation. For many legumes, rhizobial colonization initiates in root hairs through transcellular infection threads. In Medicago truncatula, VAPYRIN (VPY) and a putative E3 ligase LUMPY INFECTIONS (LIN) are required for infection thread development but their cellular and molecular roles are obscure. Here we show that LIN and its homolog LIN-LIKE interact with VPY and VPY-LIKE in a subcellular complex localized to puncta both at the tip of the growing infection thread and at the nuclear periphery in root hairs and that the punctate accumulation of VPY is positively regulated by LIN. We also show that an otherwise nuclear and cytoplasmic exocyst subunit, EXO70H4, systematically co-localizes with VPY and LIN during rhizobial infection. Genetic analysis shows that defective rhizobial infection in exo70h4 is similar to that in vpy and lin. Our results indicate that VPY, LIN and EXO70H4 are part of the symbiosis-specific machinery required for polar growth of infection threads.

Date: 2019
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DOI: 10.1038/s41467-019-10029-y

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