Tissue-specific (ts)CRISPR as an efficient strategy for in vivo screening in Drosophila

Hagar Meltzer, Efrat Marom, Idan Alyagor, Oded Mayseless, Victoria Berkun, Netta Segal-Gilboa, Tamar Unger, David Luginbuhl and Oren Schuldiner ()
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Hagar Meltzer: Weizmann Institute of Science
Efrat Marom: Weizmann Institute of Science
Idan Alyagor: Weizmann Institute of Science
Oded Mayseless: Weizmann Institute of Science
Victoria Berkun: Weizmann Institute of Science
Netta Segal-Gilboa: Weizmann Institute of Science
Tamar Unger: Weizmann Institute of Science
David Luginbuhl: Stanford University
Oren Schuldiner: Weizmann Institute of Science

Nature Communications, 2019, vol. 10, issue 1, 1-9

Abstract: Abstract Gene editing by CRISPR/Cas9 is commonly used to generate germline mutations or perform in vitro screens, but applicability for in vivo screening has so far been limited. Recently, it was shown that in Drosophila, Cas9 expression could be limited to a desired group of cells, allowing tissue-specific mutagenesis. Here, we thoroughly characterize tissue-specific (ts)CRISPR within the complex neuronal system of the Drosophila mushroom body. We report the generation of a library of gRNA-expressing plasmids and fly lines using optimized tools, which provides a valuable resource to the fly community. We demonstrate the application of our library in a large-scale in vivo screen, which reveals insights into developmental neuronal remodeling.

Date: 2019
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DOI: 10.1038/s41467-019-10140-0

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