Activated Peyer′s patch B cells sample antigen directly from M cells in the subepithelial dome

Rathan Joy Komban, Anneli Strömberg, Adi Biram, Jakob Cervin, Cristina Lebrero-Fernández, Neil Mabbott, Ulf Yrlid, Ziv Shulman, Mats Bemark () and Nils Lycke ()
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Rathan Joy Komban: University of Gothenburg
Anneli Strömberg: University of Gothenburg
Adi Biram: The Weizmann Institute of Science
Jakob Cervin: University of Gothenburg
Cristina Lebrero-Fernández: University of Gothenburg
Neil Mabbott: Edinburgh University
Ulf Yrlid: University of Gothenburg
Ziv Shulman: The Weizmann Institute of Science
Mats Bemark: University of Gothenburg
Nils Lycke: University of Gothenburg

Nature Communications, 2019, vol. 10, issue 1, 1-15

Abstract: Abstract The germinal center (GC) reaction in Peyer′s patches (PP) requires continuous access to antigens, but how this is achieved is not known. Here we show that activated antigen-specific CCR6+CCR1+GL7− B cells make close contact with M cells in the subepithelial dome (SED). Using in situ photoactivation analysis of antigen-specific SED B cells, we find migration of cells towards the GC. Following antigen injection into ligated intestinal loops containing PPs, 40% of antigen-specific SED B cells bind antigen within 2 h, whereas unspecifc cells do not, indicating B cell-receptor involvment. Antigen-loading is not observed in M cell-deficient mice, but is unperturbed in mice depleted of classical dendritic cells (DC). Thus, we report a M cell-B cell antigen-specific transporting pathway in PP that is independent of DC. We propose that this antigen transporting pathway has a critical role in gut IgA responses, and should be taken into account when developing mucosal vaccines.

Date: 2019
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DOI: 10.1038/s41467-019-10144-w

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