Anchoring cortical granules in the cortex ensures trafficking to the plasma membrane for post-fertilization exocytosis

Edgar-John Vogt, Keizo Tokuhiro, Min Guo, Ryan Dale, Guanghui Yang, Seung-Wook Shin, Maria Jimenez Movilla, Hari Shroff and Jurrien Dean ()
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Edgar-John Vogt: National Institutes of Health
Keizo Tokuhiro: National Institutes of Health
Min Guo: National Institutes of Health
Ryan Dale: National Institutes of Health
Guanghui Yang: National Institutes of Health
Seung-Wook Shin: National Institutes of Health
Maria Jimenez Movilla: University of Murcia, IMIB
Hari Shroff: National Institutes of Health
Jurrien Dean: National Institutes of Health

Nature Communications, 2019, vol. 10, issue 1, 1-13

Abstract: Abstract Following fertilization, cortical granules exocytose ovastacin, a metalloendopeptidase that cleaves ZP2 in the zona pellucida surrounding mouse eggs to prevent additional sperm binding. Using high- and super-resolution imaging with ovastacinmCherry as a fluorescent marker, we characterize cortical granule dynamics at single granule resolution in transgenic mouse eggs. Newly-developed imaging protocols provide an unprecedented view of vesicular dynamics near the plasma membrane in mouse eggs. We discover that cortical granule anchoring in the cortex is dependent on maternal MATER and document that myosin IIA is required for biphasic trafficking to the plasma membrane. We observe local clearance of cortical actin during exocytosis and determine that pharmacologic or genetic disruption of trafficking to the plasma membrane impairs secretion of cortical granules and results in polyspermy. Thus, the regulation of cortical granule dynamics at the cortex-plasma membrane interface is critical for exocytosis and the post-fertilization block to sperm binding that ensures monospermic fertilization.

Date: 2019
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DOI: 10.1038/s41467-019-10171-7

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