The Pseudomonas aeruginosa lectin LecB binds to the exopolysaccharide Psl and stabilizes the biofilm matrix

Daniel Passos da Silva, Michael L. Matwichuk, Delaney O. Townsend, Courtney Reichhardt, Doriano Lamba, Daniel J. Wozniak and Matthew R. Parsek ()
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Daniel Passos da Silva: University of Washington
Michael L. Matwichuk: University of Washington
Delaney O. Townsend: University of Washington
Courtney Reichhardt: University of Washington
Doriano Lamba: Sede Secondaria di Basovizza
Daniel J. Wozniak: Ohio State University
Matthew R. Parsek: University of Washington

Nature Communications, 2019, vol. 10, issue 1, 1-11

Abstract: Abstract Pseudomonas aeruginosa biofilms are composed of exopolysaccharides (EPS), exogenous DNA, and proteins that hold these communities together. P. aeruginosa produces lectins LecA and LecB, which possess affinities towards sugars found in matrix EPS and mediate adherence of P. aeruginosa to target host cells. Here, we demonstrate that LecB binds to Psl, a key matrix EPS, and this leads to increased retention of both cells and EPS in a growing biofilm. This interaction is predicted to occur between the lectin and the branched side chains present on Psl. Finally, we show that LecB coordinates Psl localization in the biofilm. This constitutes a unique function for LecB and identifies it as a matrix protein that contributes to biofilm structure through EPS interactions.

Date: 2019
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DOI: 10.1038/s41467-019-10201-4

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