iSuRe-Cre is a genetic tool to reliably induce and report Cre-dependent genetic modifications

Macarena Fernández-Chacón, Verónica Casquero-García, Wen Luo, Federica Francesca Lunella, Susana Ferreira Rocha, Sergio Olmo-Cabrera and Rui Benedito ()
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Macarena Fernández-Chacón: Centro Nacional de Investigaciones Cardiovasculares (CNIC)
Verónica Casquero-García: Centro Nacional de Investigaciones Cardiovasculares (CNIC)
Wen Luo: Centro Nacional de Investigaciones Cardiovasculares (CNIC)
Federica Francesca Lunella: Centro Nacional de Investigaciones Cardiovasculares (CNIC)
Susana Ferreira Rocha: Centro Nacional de Investigaciones Cardiovasculares (CNIC)
Sergio Olmo-Cabrera: Centro Nacional de Investigaciones Cardiovasculares (CNIC)
Rui Benedito: Centro Nacional de Investigaciones Cardiovasculares (CNIC)

Nature Communications, 2019, vol. 10, issue 1, 1-13

Abstract: Abstract Most biomedical research aimed at understanding gene function uses the Cre-Lox system, which consists of the Cre recombinase-dependent deletion of genes containing LoxP sites. This system enables conditional genetic modifications because the expression and activity of the recombinase Cre/CreERT2 can be regulated in space by tissue-specific promoters and in time by the ligand tamoxifen. Since the precise Cre-Lox recombination event is invisible, methods were developed to report Cre activity and are widely used. However, numerous studies have shown that expression of a given Cre activity reporter cannot be assumed to indicate deletion of other LoxP-flanked genes of interest. Here, we report the generation of an inducible dual reporter-Cre mouse allele, iSuRe-Cre. By significantly increasing Cre activity in reporter-expressing cells, iSuRe-Cre provides certainty that these cells have completely recombined floxed alleles. This genetic tool increases the ease, efficiency, and reliability of conditional mutagenesis and gene function analysis.

Date: 2019
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DOI: 10.1038/s41467-019-10239-4

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