Single particle cryo-EM reconstruction of 52 kDa streptavidin at 3.2 Angstrom resolution

Xiao Fan, Jia Wang, Xing Zhang, Zi Yang, Jin-Can Zhang, Lingyun Zhao, Hai-Lin Peng, Jianlin Lei () and Hong-Wei Wang ()
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Xiao Fan: Tsinghua University
Jia Wang: Tsinghua University
Xing Zhang: Tsinghua University
Zi Yang: Tsinghua University
Jin-Can Zhang: Peking University
Lingyun Zhao: Tsinghua University
Hai-Lin Peng: Peking University
Jianlin Lei: Tsinghua University
Hong-Wei Wang: Tsinghua University

Nature Communications, 2019, vol. 10, issue 1, 1-11

Abstract: Abstract The fast development of single-particle cryogenic electron microscopy (cryo-EM) has made it more feasible to obtain the 3D structure of well-behaved macromolecules with a molecular weight higher than 300 kDa at ~3 Å resolution. However, it remains a challenge to obtain the high-resolution structures of molecules smaller than 200 kDa using single-particle cryo-EM. In this work, we apply the Cs-corrector-VPP-coupled cryo-EM to study the 52 kDa streptavidin (SA) protein supported on a thin layer of graphene and embedded in vitreous ice. We are able to solve both the apo-SA and biotin-bound SA structures at near-atomic resolution using single-particle cryo-EM. We demonstrate that the method has the potential to determine the structures of molecules as small as 39 kDa.

Date: 2019
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DOI: 10.1038/s41467-019-10368-w

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