Importance of potassium ions for ribosome structure and function revealed by long-wavelength X-ray diffraction

Alexey Rozov (), Iskander Khusainov, Kamel El Omari, Ramona Duman, Vitaliy Mykhaylyk, Marat Yusupov, Eric Westhof, Armin Wagner () and Gulnara Yusupova ()
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Alexey Rozov: Institut de Génétique et de Biologie Moléculaire et Cellulaire
Iskander Khusainov: Institut de Génétique et de Biologie Moléculaire et Cellulaire
Kamel El Omari: Diamond Light Source, Harwell Science and Innovation Campus
Ramona Duman: Diamond Light Source, Harwell Science and Innovation Campus
Vitaliy Mykhaylyk: Diamond Light Source, Harwell Science and Innovation Campus
Marat Yusupov: Institut de Génétique et de Biologie Moléculaire et Cellulaire
Eric Westhof: Université de Strasbourg, CNRS, Architecture et Réactivité de l’ARN
Armin Wagner: Diamond Light Source, Harwell Science and Innovation Campus
Gulnara Yusupova: Institut de Génétique et de Biologie Moléculaire et Cellulaire

Nature Communications, 2019, vol. 10, issue 1, 1-12

Abstract: Abstract The ribosome, the largest RNA-containing macromolecular machinery in cells, requires metal ions not only to maintain its three-dimensional fold but also to perform protein synthesis. Despite the vast biochemical data regarding the importance of metal ions for efficient protein synthesis and the increasing number of ribosome structures solved by X-ray crystallography or cryo-electron microscopy, the assignment of metal ions within the ribosome remains elusive due to methodological limitations. Here we present extensive experimental data on the potassium composition and environment in two structures of functional ribosome complexes obtained by measurement of the potassium anomalous signal at the K-edge, derived from long-wavelength X-ray diffraction data. We elucidate the role of potassium ions in protein synthesis at the three-dimensional level, most notably, in the environment of the ribosome functional decoding and peptidyl transferase centers. Our data expand the fundamental knowledge of the mechanism of ribosome function and structural integrity.

Date: 2019
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DOI: 10.1038/s41467-019-10409-4

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