Coherent diffractive imaging of microtubules using an X-ray laser

Gisela Brändén (), Greger Hammarin, Rajiv Harimoorthy, Alexander Johansson, David Arnlund, Erik Malmerberg, Anton Barty, Stefan Tångefjord, Peter Berntsen, Daniel P. DePonte, Carolin Seuring, Thomas A. White, Francesco Stellato, Richard Bean, Kenneth R. Beyerlein, Leonard M. G. Chavas, Holger Fleckenstein, Cornelius Gati, Umesh Ghoshdastider, Lars Gumprecht, Dominik Oberthür, David Popp, Marvin Seibert, Thomas Tilp, Marc Messerschmidt, Garth J. Williams, N. Duane Loh, Henry N. Chapman, Peter Zwart, Mengning Liang, Sébastien Boutet, Robert C. Robinson and Richard Neutze ()
Additional contact information
Gisela Brändén: University of Gothenburg
Greger Hammarin: University of Gothenburg
Rajiv Harimoorthy: University of Gothenburg
Alexander Johansson: University of Gothenburg
David Arnlund: University of Gothenburg
Erik Malmerberg: Lawrence Berkeley National Laboratory
Anton Barty: Deutsches Elektronen-Synchrotron DESY
Stefan Tångefjord: University of Gothenburg
Peter Berntsen: University of Gothenburg
Daniel P. DePonte: SLAC National Accelerator Laboratory
Carolin Seuring: Deutsches Elektronen-Synchrotron DESY
Thomas A. White: Deutsches Elektronen-Synchrotron DESY
Francesco Stellato: Deutsches Elektronen-Synchrotron DESY
Richard Bean: Deutsches Elektronen-Synchrotron DESY
Kenneth R. Beyerlein: Deutsches Elektronen-Synchrotron DESY
Leonard M. G. Chavas: Deutsches Elektronen-Synchrotron DESY
Holger Fleckenstein: Deutsches Elektronen-Synchrotron DESY
Cornelius Gati: Deutsches Elektronen-Synchrotron DESY
Umesh Ghoshdastider: Biopolis, A*STAR (Agency for Science, Technology and Research)
Lars Gumprecht: Deutsches Elektronen-Synchrotron DESY
Dominik Oberthür: Deutsches Elektronen-Synchrotron DESY
David Popp: Biopolis, A*STAR (Agency for Science, Technology and Research)
Marvin Seibert: SLAC National Accelerator Laboratory
Thomas Tilp: Deutsches Elektronen-Synchrotron DESY
Marc Messerschmidt: SLAC National Accelerator Laboratory
Garth J. Williams: SLAC National Accelerator Laboratory
N. Duane Loh: National University of Singapore
Henry N. Chapman: Deutsches Elektronen-Synchrotron DESY
Peter Zwart: Lawrence Berkeley National Laboratory
Mengning Liang: Deutsches Elektronen-Synchrotron DESY
Sébastien Boutet: SLAC National Accelerator Laboratory
Robert C. Robinson: Biopolis, A*STAR (Agency for Science, Technology and Research)
Richard Neutze: University of Gothenburg

Nature Communications, 2019, vol. 10, issue 1, 1-9

Abstract: Abstract X-ray free electron lasers (XFELs) create new possibilities for structural studies of biological objects that extend beyond what is possible with synchrotron radiation. Serial femtosecond crystallography has allowed high-resolution structures to be determined from micro-meter sized crystals, whereas single particle coherent X-ray imaging requires development to extend the resolution beyond a few tens of nanometers. Here we describe an intermediate approach: the XFEL imaging of biological assemblies with helical symmetry. We collected X-ray scattering images from samples of microtubules injected across an XFEL beam using a liquid microjet, sorted these images into class averages, merged these data into a diffraction pattern extending to 2 nm resolution, and reconstructed these data into a projection image of the microtubule. Details such as the 4 nm tubulin monomer became visible in this reconstruction. These results illustrate the potential of single-molecule X-ray imaging of biological assembles with helical symmetry at room temperature.

Date: 2019
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DOI: 10.1038/s41467-019-10448-x

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