MRI-guided robotic arm drives optogenetic fMRI with concurrent Ca2+ recording

Yi Chen, Patricia Pais-Roldan, Xuming Chen, Michael H. Frosz and Xin Yu ()
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Yi Chen: Max Planck Institute for Biological Cybernetics
Patricia Pais-Roldan: Max Planck Institute for Biological Cybernetics
Xuming Chen: Max Planck Institute for Biological Cybernetics
Michael H. Frosz: Max Planck Institute for the Science of Light
Xin Yu: Max Planck Institute for Biological Cybernetics

Nature Communications, 2019, vol. 10, issue 1, 1-11

Abstract: Abstract Optical fiber-mediated optogenetic activation and neuronal Ca2+ recording in combination with fMRI provide a multi-modal fMRI platform. Here, we developed an MRI-guided robotic arm (MgRA) as a flexible positioning system with high precision to real-time assist optical fiber brain intervention for multi-modal animal fMRI. Besides the ex vivo precision evaluation, we present the highly reliable brain activity patterns in the projected basal forebrain regions upon MgRA-driven optogenetic stimulation in the lateral hypothalamus. Also, we show the step-wise optical fiber targeting thalamic nuclei and map the region-specific functional connectivity with whole-brain fMRI accompanied by simultaneous calcium recordings to specify its circuit-specificity. The MgRA also guides the real-time microinjection to specific deep brain nuclei, which is demonstrated by an Mn-enhanced MRI method. The MgRA represents a clear advantage over the standard stereotaxic-based fiber implantation and opens a broad avenue to investigate the circuit-specific functional brain mapping with the multi-modal fMRI platform.

Date: 2019
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DOI: 10.1038/s41467-019-10450-3

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