Multi-immersion open-top light-sheet microscope for high-throughput imaging of cleared tissues

Adam K. Glaser (), Nicholas P. Reder, Ye Chen, Chengbo Yin, Linpeng Wei, Soyoung Kang, Lindsey A. Barner, Weisi Xie, Erin F. McCarty, Chenyi Mao, Aaron R. Halpern, Caleb R. Stoltzfus, Jonathan S. Daniels, Michael Y. Gerner, Philip R. Nicovich, Joshua C. Vaughan, Lawrence D. True and Jonathan T. C. Liu ()
Additional contact information
Adam K. Glaser: University of Washington
Nicholas P. Reder: University of Washington
Ye Chen: University of Washington
Chengbo Yin: University of Washington
Linpeng Wei: University of Washington
Soyoung Kang: University of Washington
Lindsey A. Barner: University of Washington
Weisi Xie: University of Washington
Erin F. McCarty: University of Washington
Chenyi Mao: University of Washington
Aaron R. Halpern: University of Washington
Caleb R. Stoltzfus: University of Washington
Jonathan S. Daniels: Applied Scientific Instrumentation
Michael Y. Gerner: University of Washington
Philip R. Nicovich: Allen Institute for Brain Science
Joshua C. Vaughan: University of Washington
Lawrence D. True: University of Washington
Jonathan T. C. Liu: University of Washington

Nature Communications, 2019, vol. 10, issue 1, 1-8

Abstract: Abstract Recent advances in optical clearing and light-sheet microscopy have provided unprecedented access to structural and molecular information from intact tissues. However, current light-sheet microscopes have imposed constraints on the size, shape, number of specimens, and compatibility with various clearing protocols. Here we present a multi-immersion open-top light-sheet microscope that enables simple mounting of multiple specimens processed with a variety of clearing protocols, which will facilitate wide adoption by preclinical researchers and clinical laboratories. In particular, the open-top geometry provides unsurpassed versatility to interface with a wide range of accessory technologies in the future.

Date: 2019
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DOI: 10.1038/s41467-019-10534-0

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