A quantitative Streptococcus pyogenes–human protein–protein interaction map reveals localization of opsonizing antibodies

Lotta Happonen, Simon Hauri, Gabriel Svensson Birkedal, Christofer Karlsson, Therese Neergaard, Hamed Khakzad, Pontus Nordenfelt, Mats Wikström, Magdalena Wisniewska, Lars Björck, Lars Malmström and Johan Malmström ()
Additional contact information
Lotta Happonen: Lund University
Simon Hauri: Lund University
Gabriel Svensson Birkedal: Lund University
Christofer Karlsson: Lund University
Therese Neergaard: Lund University
Hamed Khakzad: University of Zurich
Pontus Nordenfelt: Lund University
Mats Wikström: University of Copenhagen
Magdalena Wisniewska: University of Copenhagen
Lars Björck: Lund University
Lars Malmström: Lund University
Johan Malmström: Lund University

Nature Communications, 2019, vol. 10, issue 1, 1-15

Abstract: Abstract A fundamental challenge in medical microbiology is to characterize the dynamic protein–protein interaction networks formed at the host–pathogen interface. Here, we generate a quantitative interaction map between the significant human pathogen, Streptococcus pyogenes, and proteins from human saliva and plasma obtained via complementary affinity-purification and bacterial-surface centered enrichment strategies and quantitative mass spectrometry. Perturbation of the network using immunoglobulin protease cleavage, mixtures of different concentrations of saliva and plasma, and different S. pyogenes serotypes and their isogenic mutants, reveals how changing microenvironments alter the interconnectivity of the interaction map. The importance of host immunoglobulins for the interaction with human complement proteins is demonstrated and potential protective epitopes of importance for phagocytosis of S. pyogenes cells are localized. The interaction map confirms several previously described protein–protein interactions; however, it also reveals a multitude of additional interactions, with possible implications for host–pathogen interactions involving other bacterial species.

Date: 2019
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-10583-5

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DOI: 10.1038/s41467-019-10583-5

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